In vitro propagation of Solidago virgaurea L. through nodal culture

A reliable in vitro propagation protocol has been established from nodal segments of a highly valuable medicinal plant Solidago virgaurea L. The explants were cultured on Murashige and Skoog’s medium augmented with different concentrations and combinations of plant growth regulators for shoot bud initiation and multiplications. Highest frequency of shoot proliferation (91.8 ± 0.64) and maximum number (12.1 ± 0.20) per node was observed in Murashige and Skoog’s medium augmented with4.5 mg/l of Kinetin in combination with 0.5 mg/l of Indole -3Acetic acid. Highest frequency (91.7 ± 1.43) of rooting and maximum number (3.8 ± 0.28) of rootlet per shoot let was achieved on Murashige and Skoog’s medium fortified with 4.5 mg/l of Indole -3Acetic acid in combination with 0.5 mg/l of Benzyl -6amino purine. Regenerated plants were successfully transferred to field (78%).